95COMPARISION OF IN-VITRO ANTIDIABETIC SCREENING METHODS FOR CERTAIN SYNTHESIZED 4-PHENYL-6-SUBSTITUTED PHENYL-3,4-DIHYDRO PYRIMIDINE-2-ONE AND N-PHENYL-3-PHENYL-5- SUBSTITUTED PHENYL PYRAZOLINE ANALOGUESTwo series of heterocyclic analogues were synthesized from substituted chalcones (C1-5) which was prepared by reaction of acetophenone (a) and aromatic aldehydes (b1-5). One series of N-phenyl pyrazoline derivatives (K1-K5) were synthesized by reaction of chalcones (C1-5) with phenyl hydrazine in acidic medium. Another series of 3,4-dihydropyrimidine-2-one derivatives (K6-K10) were synthesized by reaction of chalcones (C1-5) with ethanolic urea in alkali medium. The yield of the synthesized compounds ranged from 62-76%. The In-vitro antidiabetic screening was performed by non enzymatic glycosylation of haemoglobin assay, α-amylase enzyme inhibition assay, α-Glucosidase enzyme inhibition assay methods. The result of all heterocyclic analogues (K1-K10) indicates that have pronounced potency when compared to standard drugs. Among that analogues K1 & K6 showed nearly equal action and analogues K2 & K7 also showed appreciable activity when compared to the standard drugs at 50μg/ml, 100μg/ml, and 150μg/ml tested concentrations.drselvakumar69@gmail.comResearch622016Pyrazoline,Pyrimidine,Chalcone,α-amylase,α-glucosidase,anti-diabeticS.Selvakumar,R.Nallathambi,L.Matsyagiri,P.Prapulla,Afreen,P.PranathiSwami Vivekananda Institute of Pharmaceutical Sciences, Vangapally (V), Yadagirigutta (M), Nalgonda (Dt), Telangana –508 286, India.,Sri Kakatiya Institute of Pharmaceutical Sciences, Unikicherla, Dharamsagar (M), Warangal, Telangana –506370, India.,Swami Vivekananda Institute of Pharmaceutical Sciences, Vangapally (V), Yadagirigutta (M), Nalgonda (Dt), Telangana –508 286, India.,Swami Vivekananda Institute of Pharmaceutical Sciences, Vangapally (V), Yadagirigutta (M), Nalgonda (Dt), Telangana –508 286, India.,Swami Vivekananda Institute of Pharmaceutical Sciences, Vangapally (V), Yadagirigutta (M), Nalgonda (Dt), Telangana –508 286, India.,Swami Vivekananda Institute of Pharmaceutical Sciences, Vangapally (V), Yadagirigutta (M), Nalgonda (Dt), Telangana –508 286, India.